Tbru glycolytic enzymes 6-12-09

Posted by Greg Crowther on 2009-06-12 23:08:55

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Consider the following scenario: you're interested in the glycolytic pathway, which has been validated as a target in T. brucei (e.g., PMID 15955817). Which enzyme(s) should you investigate further? Could do a prioritization of glycolytic/gluconeogenic enzymes with the following criteria:
* KEGG glycolysis/gluconeogenesis
* weighted union including
- PDB structures - 20
- Modbase models - 10
- assayability - 20
- literature references - 20
- druggability > 0.4 - 20
- compound desirability > 0.2 - 10
- number of paralogs = 1 - 10
* additional points awarded in proportion to flux control according to PMID 15955817:
- GAPDH (1.2.1.12) – 40
- glycerol-3-P DH (1.1.1.8) – 30
- glycerol-3-phosphate oxidase (1.1.99.5) – 30
- PGAM (5.4.2.1) – 30
- aldolase (4.1.2.13) – 10
- enolase (4.2.1.11) – 10
- PGK (2.7.2.3) – 10
- pyruvate kinase (2.7.1.40) – 10

● Experimental and in silico analyses of glycolytic flux control in bloodstream form Trypanosoma brucei. (2005). J Biol Chem 280: 28306-15 PubMed, Publisher.

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  Union of (397, 398) (33 records). Show parameters.