Detailed view for Tb927.9.9960

Basic information

TDR Targets ID: 12835
Trypanosoma brucei, katanin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0438 | Length (AA): 538 | MW (Da): 59648 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat
PF13925   con80 domain of Katanin

Gene Ontology

Mouse over links to read term descriptions.
GO:0003674   molecular_function  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129945)

Species Accession Gene Product
Arabidopsis thaliana AT5G08390   transducin/WD40 repeat-like superfamily protein
Arabidopsis thaliana AT1G61210   protein DWD hypersensitive to ABA 3
Arabidopsis thaliana AT5G23430   Katanin p80 WD40 repeat-containing subunit B1-1
Arabidopsis thaliana AT1G11160   WD40 domain-containing protein
Drosophila melanogaster Dmel_CG13956   katanin 80
Echinococcus granulosus EgrG_000480700   katanin p80 WD40 containing subunit B1
Echinococcus multilocularis EmuJ_000480700   katanin p80 WD40 containing subunit B1
Homo sapiens ENSG00000140854   katanin p80 (WD repeat containing) subunit B 1
Leishmania braziliensis LbrM.34.4650   katanin, putative
Leishmania donovani LdBPK_354740.1   katanin, putative
Leishmania infantum LinJ.35.4740   katanin, putative
Leishmania major LmjF.35.4670   katanin, putative
Leishmania mexicana LmxM.34.4670   katanin, putative
Mus musculus ENSMUSG00000031787   katanin p80 (WD40-containing) subunit B 1
Oryza sativa 4348995   Os10g0494800
Oryza sativa 4327835   Os01g0780400
Oryza sativa 4337408   Os04g0677700
Schistosoma japonicum Sjp_0087640   Katanin p80 WD40-containing subunit B1, putative
Schmidtea mediterranea mk4.009235.03   Putative katanin P80 subunit
Schmidtea mediterranea mk4.009235.04   Putative katanin P80 subunit
Schmidtea mediterranea mk4.010406.00   Putative katanin P80 subunit
Trypanosoma brucei gambiense Tbg972.9.5740   katanin, putative
Trypanosoma brucei Tb927.9.9960   katanin, putative
Trypanosoma congolense TcIL3000_9_3630   katanin, putative
Trypanosoma cruzi TcCLB.506887.50   katanin, putative
Trypanosoma cruzi TcCLB.510731.40   katanin, putative
Trichomonas vaginalis TVAG_130970   G-protein beta WD- 40 repeats containing protein, putative
Trichomonas vaginalis TVAG_397060   katanin P80 subunit, putative
Trichomonas vaginalis TVAG_230250   WD-repeat protein, putative
Trichomonas vaginalis TVAG_499000   katanin P80 subunit, putative
Trichomonas vaginalis TVAG_363890   katanin P80 subunit, putative
Trichomonas vaginalis TVAG_165010   katanin P80 subunit, putative

Essentiality

Tb927.9.9960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1500 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.1500 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.1500 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.1500 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.9960 (Trypanosoma brucei), katanin, putative
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