Detailed view for PF3D7_1354400

Basic information

TDR Targets ID: 186
Plasmodium falciparum, V-type proton ATPase 21 kDa proteolipid subunit, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.0781 | Length (AA): 181 | MW (Da): 19124 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00137   ATP synthase subunit C

Gene Ontology

Mouse over links to read term descriptions.
GO:0033179   proton-transporting V-type ATPase, V0 domain  
GO:0033177   proton-transporting two-sector ATPase complex, proton-transporting domain  
GO:0016471   vacuolar proton-transporting V-type ATPase complex  
GO:0015078   hydrogen ion transmembrane transporter activity  
GO:0015991   ATP hydrolysis coupled proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 178 2bl2 (A) 4 156 16.00 0 0.05 1.11 -1.3
117 173 4bem (A) 11 74 39.00 0.17 0.04 0.406517 0.97

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127898)

Species Accession Gene Product
Arabidopsis thaliana AT2G25610   ATPase, F0/V0 complex, subunit C protein
Arabidopsis thaliana AT4G32530   ATPase, F0/V0 complex, subunit C protein
Babesia bovis BBOV_III009220   ATP synthase subunit C domain containing protein
Brugia malayi Bm1_43140   Vacuolar ATP synthase 21 kDa proteolipid subunit, putative
Candida albicans CaO19.4954   Proteolipid - vacuolar ATPase V0 domain subunit c''
Candida albicans CaO19.12419   c subunit of V0 domain of V-ATPase
Caenorhabditis elegans CELE_T01H3.1   Protein VHA-4
Cryptosporidium hominis Chro.80551   V-ATPase subunit c proteolipid
Cryptosporidium parvum cgd8_4790   proteolipid subunit of the vacuolar ATpase
Dictyostelium discoideum DDB_G0274141   hypothetical protein
Drosophila melanogaster Dmel_CG7026   Vacuolar H[+] ATPase PPA1 subunit 2
Drosophila melanogaster Dmel_CG7007   Vacuolar H[+] ATPase PPA1 subunit 1
Echinococcus granulosus EgrG_001145000   V type proton ATPase 21 kDa proteolipid subunit
Entamoeba histolytica EHI_029370   Vacuolar ATP synthase proteolipid subunit, putative
Echinococcus multilocularis EmuJ_001145000   V type proton ATPase 21 kDa proteolipid subunit
Giardia lamblia GL50803_15598   Vacuolar ATP synthase 16 kDa proteolipid subunit
Homo sapiens ENSG00000117410   ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
Leishmania braziliensis LbrM.30.3690   V-type ATPase, C subunit, putative
Leishmania donovani LdBPK_303720.1   V-type ATPase, C subunit, putative
Leishmania infantum LinJ.30.3720   V-type ATPase, C subunit, putative
Leishmania major LmjF.30.3660   V-type ATPase, C subunit, putative
Leishmania mexicana LmxM.29.3660   V-type ATPase, C subunit, putative
Loa Loa (eye worm) LOAG_05327   vacuolar ATP synthase proteolipid subunit
Mus musculus ENSMUSG00000033379   ATPase, H+ transporting, lysosomal V0 subunit B
Neospora caninum NCLIV_043040   GE13384, related
Oryza sativa 4327731   Os01g0610100
Plasmodium berghei PBANKA_1131000   V-type proton ATPase 21 kDa proteolipid subunit, putative
Plasmodium falciparum PF3D7_1354400   V-type proton ATPase 21 kDa proteolipid subunit, putative
Plasmodium knowlesi PKNH_1117400   V-type proton ATPase 21 kDa proteolipid subunit, putative
Plasmodium vivax PVX_114705   V-type proton ATPase 21 kDa proteolipid subunit, putative
Plasmodium yoelii PY05899   ATP synthase subunit C, putative
Saccharomyces cerevisiae YHR026W   H(+)-transporting V0 sector ATPase subunit c''
Schistosoma japonicum Sjp_0036000   ko:K03661 V-type H+-transporting ATPase 21kDa proteolipid subunit, putative
Schistosoma mansoni Smp_064350   vacuolar ATP synthase proteolipid subunit
Schmidtea mediterranea mk4.001760.02   V-type proton ATPase 21 kDa proteolipid subunit
Trypanosoma brucei gambiense Tbg972.6.4850   V-type ATPase, C subunit, putative
Trypanosoma brucei Tb927.6.5050   V-type ATPase, C subunit, putative
Trypanosoma congolense TcIL3000_6_4480   V-type ATPase, C subunit, putative
Trypanosoma cruzi TcCLB.506945.300   V-type ATPase, C subunit, putative
Toxoplasma gondii TGME49_291310   V-ATPase subunit c' proteolipid, putative
Theileria parva TP04_0832   vacuolar ATP synthase subunit C, putative
Trichomonas vaginalis TVAG_371960   vacuolar ATP synthase proteolipid subunit 1, 2, 3, putative

Essentiality

PF3D7_1354400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.5050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.5050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.5050 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.6.5050 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T01H3.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T01H3.1 Caenorhabditis elegans larval lethal wormbase
CELE_T01H3.1 Caenorhabditis elegans sterile wormbase
PBANKA_1131000 Plasmodium berghei Essential plasmo
TGME49_291310 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_1354400 (Plasmodium falciparum), V-type proton ATPase 21 kDa proteolipid subunit, putative
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