Detailed view for TGME49_313450

Basic information

TDR Targets ID: 257641
Toxoplasma gondii, AP-2 complex subunit sigma-1, putative
Source Database / ID:  ToxoDB 

pI: 7.839 | Length (AA): 143 | MW (Da): 16781 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01217   Clathrin adaptor complex small chain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030117   membrane coat  
GO:0005515   protein binding  
GO:0008565   protein transporter activity  
GO:0016192   vesicle-mediated transport  
GO:0015031   protein transport  
GO:0006886   intracellular protein transport  
GO:0006810   transport  

Metabolic Pathways

Endocytosis (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 143 1gw5 (S) 1 142 44.00 0 1 1.71 -2.41
1 143 2vgl (S) 1 142 44.00 0 1 1.7068 -1.75
1 143 4hmy (S) 1 142 45.00 0 1 1.6505 -1.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128581)

Species Accession Gene Product
Arabidopsis thaliana AT1G47830   SNARE-like superfamily protein
Babesia bovis BBOV_III005320   clathrin adaptor complex small chain family protein
Brugia malayi Bm1_18635   clathrin adaptor complex small chain
Candida albicans CaO19.1136   similar to S. cerevisiae APS2 (YJR058C) small subunit of the clathrin-associated protein AP-2 complex
Candida albicans CaO19.8729   similar to S. cerevisiae APS2 (YJR058C) small subunit of the clathrin-associated protein AP-2 complex
Caenorhabditis elegans CELE_F02E8.3   Protein APS-2
Dictyostelium discoideum DDB_G0289721   adaptor-related protein complex 2, sigma 1 subunit
Drosophila melanogaster Dmel_CG6056   Adaptor Protein complex 2, sigma subunit
Echinococcus granulosus EgrG_000065450   clathrin coat assembly protein ap17
Entamoeba histolytica EHI_009940   clathrin adaptor complex small chain, putative
Echinococcus multilocularis EmuJ_000065450   clathrin coat assembly protein ap17
Giardia lamblia GL50803_5328   Sigma adaptin
Homo sapiens ENSG00000042753   adaptor-related protein complex 2, sigma 1 subunit
Leishmania braziliensis LbrM.20.1830   clathrin coat assembly protein AP17, putative
Leishmania donovani LdBPK_342100.1   clathrin coat assembly protein AP17, putative
Leishmania infantum LinJ.34.2100   clathrin coat assembly protein AP17, putative
Leishmania major LmjF.34.2330   clathrin coat assembly protein AP17, putative
Leishmania mexicana LmxM.33.2330   clathrin coat assembly protein AP17, putative
Loa Loa (eye worm) LOAG_08185   clathrin adaptor complex small chain
Mus musculus ENSMUSG00000008036   adaptor-related protein complex 2, sigma 1 subunit
Neospora caninum NCLIV_056750   Ap4s1 protein, related
Oryza sativa 4351755   Os12g0207300
Plasmodium berghei PBANKA_0314000   AP-2 complex subunit sigma, putative
Plasmodium falciparum PF3D7_0217300   AP-2 complex subunit sigma, putative
Plasmodium knowlesi PKNH_0403600   AP-2 complex subunit sigma, putative
Plasmodium vivax PVX_002675   AP-2 complex subunit sigma, putative
Plasmodium yoelii PY01700   hypothetical protein
Saccharomyces cerevisiae YJR058C   Aps2p
Schistosoma japonicum Sjp_0086800   AP-2 complex subunit sigma-1, putative
Schistosoma mansoni Smp_143060   clathrin coat assembly protein AP17
Trypanosoma cruzi TcCLB.506559.330   clathrin coat assembly protein AP17, putative
Toxoplasma gondii TGME49_313450   AP-2 complex subunit sigma-1, putative
Theileria parva TP02_0416   clathrin assembly protein, putative
Trichomonas vaginalis TVAG_134680   clathrin coat assembly protein ap19, putative
Trichomonas vaginalis TVAG_262470   clathrin coat assembly protein ap19, putative
Trichomonas vaginalis TVAG_214290   clathrin coat assembly protein ap17, putative

Essentiality

TGME49_313450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F02E8.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_F02E8.3 Caenorhabditis elegans larval lethal wormbase
CELE_F02E8.3 Caenorhabditis elegans sterile wormbase
TGME49_313450 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus AP-2 complex subunit sigma Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0032 0.5 0.5
0.0033 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_313450 (Toxoplasma gondii), AP-2 complex subunit sigma-1, putative
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