Detailed view for TGME49_286778

Basic information

TDR Targets ID: 258009
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 6.8388 | Length (AA): 190 | MW (Da): 20954 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0048038   quinone binding  
GO:0008131   amine oxidase activity  
GO:0005507   copper ion binding  
GO:0055114   oxidation reduction  
GO:0009308   amine metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
59 156 1gxe (A) 35 122 31.00 0.69 0.17 0.631689 0.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127171)

Species Accession Gene Product
Arabidopsis thaliana AT1G31710   copper amine oxidase 2
Arabidopsis thaliana AT4G14940   amine oxidase 1
Arabidopsis thaliana AT4G12290   copper amine oxidase family protein
Arabidopsis thaliana AT1G31670   copper amine oxidase family protein
Arabidopsis thaliana AT4G12270   copper amine oxidase family protein
Arabidopsis thaliana AT2G42490   copper amine oxidase family protein
Arabidopsis thaliana AT1G62810   copper amine oxidase 1
Arabidopsis thaliana AT1G31690   copper amine oxidase family protein
Arabidopsis thaliana AT3G43670   putative copper amine oxidase
Candida albicans CaO19.3152   one of two likely peroxisomal copper amine oxidase genes
Candida albicans CaO19.5784   one of two likely copper amine oxidase genes
Candida albicans CaO19.10662   one of two likely peroxisomal copper amine oxidase genes
Candida albicans CaO19.13206   one of two likely copper amine oxidase genes
Cryptosporidium hominis Chro.30388   amiloride binding protein 1
Cryptosporidium parvum cgd3_3430   extracellular protein with a signal peptide sequence, MAM domain and a Cu amine oxidase domain
Escherichia coli b1386   tyramine oxidase, copper-requiring
Echinococcus granulosus EgrG_000530400   amiloride sensitive amine oxidase
Echinococcus multilocularis EmuJ_000530400   amiloride sensitive amine oxidase
Homo sapiens 102724175   membrane primary amine oxidase-like
Homo sapiens ENSG00000002726   amine oxidase, copper containing 1
Homo sapiens ENSG00000131480   amine oxidase, copper containing 2 (retina-specific)
Homo sapiens ENSG00000131471   amine oxidase, copper containing 3
Mus musculus ENSMUSG00000029813   RIKEN cDNA 1600015I10 gene
Mus musculus ENSMUSG00000078651   amine oxidase, copper containing 2 (retina-specific)
Mus musculus ENSMUSG00000068536   diamine oxidase-like protein 2
Mus musculus ENSMUSG00000019326   amine oxidase, copper containing 3
Mus musculus ENSMUSG00000029811   amine oxidase, copper-containing 1
Neospora caninum NCLIV_013880   hypothetical protein
Oryza sativa 4335335   Os04g0269600
Oryza sativa 4336153   Os04g0476100
Oryza sativa 4343673   Os07g0572100
Oryza sativa 4340946   Os06g0338700
Oryza sativa 4340945   Os06g0338200
Schistosoma japonicum Sjp_0007180   ko:K00276 copper amine oxidase [EC1.4.3.6], putative
Schistosoma japonicum Sjp_0007190   IPR000269,Copper amine oxidase,domain-containing
Schistosoma mansoni Smp_017610   copper/topaquinone oxidase
Schistosoma mansoni Smp_017620   hypothetical protein
Schmidtea mediterranea mk4.000236.15   Amine oxidase
Toxoplasma gondii TGME49_286778   hypothetical protein

Essentiality

TGME49_286778 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b1386 Escherichia coli non-essential goodall
TGME49_286778 this record Toxoplasma gondii Probably non-essential sidik
TGME49_286778 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Amine oxidase, copper containing Compounds References
Homo sapiens amine oxidase, copper containing 2 (retina-specific) Compounds References
Rattus norvegicus Diamine oxidase Compounds References
Bos taurus Amine oxidase, copper containing Compounds References
Homo sapiens amine oxidase, copper containing 3 Compounds References
Mus musculus amine oxidase, copper containing 3 Compounds References
Homo sapiens amine oxidase, copper containing 1 Compounds References
Pisum sativum Amine oxidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0652 0.4754 0.5
0.041 0.4694 1
0.0794 0.4975 1
0.0104 0.2624 1
0.0183 0.4323 1
0.032 0.4695 1
0.0663 0.5003 0.5
0.0477 0.295 1
0.0333 0.4886 0.5
0.1201 0.3571 1
0.0464 0.4926 1
0.0663 0.5003 0.5
0.0182 0.3138 1
0.0097 0.3146 1
0.0418 0.4883 1
0.0277 0.2765 1
0.0126 0.4009 0.5
0.0533 0.3118 1
0.0275 0.3018 0.3129
0.0309 0.3957 0.5
0.0267 0.4608 1
0.0181 0.348 1
0.0422 0.4836 1
0.0266 0.4709 0.5
0.0279 0.3378 0.5
0.0179 0.4359 1
0.1164 0.5042 1
0.032 0.4695 1
0.0371 0.4234 0.5
0.0347 0.4115 1
0.032 0.4695 1
0.0951 0.3263 1
0.048 0.5025 1
0.0104 0.2624 1
0.064 0.4981 1
0.0086 0.3614 0.5
0.0755 0.4523 0.5
0.0104 0.314 0.3289
0.0362 0.4093 1
0.0445 0.4859 1
0.0649 0.4827 1
0.0783 0.4476 0.5
0.0952 0.4992 0.7188
0.0312 0.4816 0.5
0.0047 0.2822 0.3746
0.0524 0.4192 0.5
0.0052 0.385 1
0.0413 0.4963 1
0.0365 0.3464 1
0.0275 0.3018 0.3129
0.0104 0.4922 1
0.0649 0.4827 1
0.013 0.4326 0.5
0.0286 0.4506 0.5
0.0598 0.4877 1
0.0649 0.4827 1
0.0052 0.2606 1
0.0683 0.2547 1
0.012 0.2677 1
0.0181 0.348 1
0.0256 0.4463 0.5
0.0683 0.2547 1
0.0277 0.2765 1
0.0316 0.4978 1
0.0186 0.4385 1
0.0637 0.4928 1
0.0368 0.3353 0.5
0.0096 0.4783 1
0.0445 0.4859 1
0.0637 0.4928 1
0.009 0.2615 1
0.0598 0.4877 1
0.0052 0.385 1
0.0603 0.4016 0.5
0.0222 0.4707 1
0.0743 0.4844 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_286778 (Toxoplasma gondii), hypothetical protein
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