Detailed view for PF3D7_0212300

Basic information

TDR Targets ID: 3387
Plasmodium falciparum, peptide chain release factor subunit 1, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.9715 | Length (AA): 427 | MW (Da): 48064 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03463   eRF1 domain 1
PF03464   eRF1 domain 2
PF03465   eRF1 domain 3

Gene Ontology

Mouse over links to read term descriptions.
GO:0006449   regulation of translational termination  
GO:0005737   cytoplasm  
GO:0003747   translation release factor activity  
GO:0006415   translational termination  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 415 1dt9 (A) 6 421 72.00 0 1 1.71 -0.69
2 415 1dt9 (A) 6 421 72.00 0 1 1.72575 -0.24
4 422 3e20 (C) 5 427 57.00 0 1 0.789565 2.46
4 412 3e1y (C) 8 418 70.00 0 1 1.42015 0.42
273 385 2fc3 (A) 6 127 21.00 0.95 0.18 0.450737 -0.37

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late trophozoite, Oocyst, Ring, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile late schizont, Male Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127250)

Species Accession Gene Product
Arabidopsis thaliana AT1G12920   eukaryotic peptide chain release factor subunit 1-2
Arabidopsis thaliana AT3G26618   peptide chain release factor eRF subunit 1
Arabidopsis thaliana AT5G47880   peptide chain release factor eRF subunit 1
Babesia bovis BBOV_IV003460   peptide chain release factor subunit 1
Brugia malayi Bm1_53065   Eukaryotic peptide chain release factor subunit 1, putative
Candida albicans CaO19.11025   translation release factor eRF1
Candida albicans CaO19.3541   translation release factor eRF1
Caenorhabditis elegans CELE_T05H4.6   Protein ETF-1
Cryptosporidium hominis Chro.60391   eukaryotic peptide chain release factor
Cryptosporidium parvum cgd6_3380   Erf1 eukaryotic translation termination factor 1; N-terminal RNAseH plus pelota domain containing protein
Dictyostelium discoideum DDB_G0288613   eukaryotic petide chain release factor subunit 1
Drosophila melanogaster Dmel_CG5605   eukaryotic release factor 1
Echinococcus granulosus EgrG_000801200   eukaryotic peptide chain release factor subunit
Entamoeba histolytica EHI_152750   eukaryotic peptide chain release factor subunit 1, putative
Echinococcus multilocularis EmuJ_000801200   eukaryotic peptide chain release factor subunit
Giardia lamblia GL50803_17190   Eukaryotic peptide chain release factor subunit 1
Homo sapiens ENSG00000120705   eukaryotic translation termination factor 1
Leishmania braziliensis LbrM.27.1850   eukaryotic translation release factor, putative
Leishmania donovani LdBPK_271610.1   eukaryotic translation release factor, putative
Leishmania infantum LinJ.27.1610   eukaryotic translation release factor, putative
Leishmania mexicana LmxM.27.1710   eukaryotic translation release factor, putative
Loa Loa (eye worm) LOAG_09139   eukaryotic peptide chain release factor subunit 1
Mus musculus ENSMUSG00000024360   eukaryotic translation termination factor 1
Neospora caninum NCLIV_044440   hypothetical protein
Oryza sativa 4326511   Os01g0939500
Oryza sativa 4338603   Os05g0373900
Oryza sativa 4343757   Os07g0587400
Oryza sativa 4333840   Os03g0702800
Onchocerca volvulus OVOC6237   Eukaryotic peptide chain release factor subunit 1 homolog
Onchocerca volvulus OVOC6241   Eukaryotic peptide chain release factor subunit 1 homolog
Plasmodium berghei PBANKA_0309100   peptide chain release factor subunit 1, putative
Plasmodium falciparum PF3D7_0212300   peptide chain release factor subunit 1, putative
Plasmodium knowlesi PKNH_0408900   peptide chain release factor subunit 1, putative
Plasmodium vivax PVX_002915   eukaryotic peptide chain release factor subunit 1, putative
Plasmodium yoelii PY03558   peptide chain release factor eRF/aRF, subunit 1
Saccharomyces cerevisiae YBR143C   translation termination factor eRF1
Schistosoma japonicum Sjp_0005880   ko:K03265 peptide chain release factor eRF subunit 1, putative
Schistosoma mansoni Smp_066630.2   eukaryotic peptide chain release factor subunit
Schistosoma mansoni Smp_066630.1   eukaryotic peptide chain release factor subunit
Schmidtea mediterranea mk4.004120.01   Eukaryotic peptide chain release factor subunit 1
Schmidtea mediterranea mk4.000384.00  
Schmidtea mediterranea mk4.004890.01  
Schmidtea mediterranea mk4.004904.01  
Trypanosoma brucei gambiense Tbg972.11.2550   eukaryotic peptide chain release factor subunit 1, putative
Trypanosoma brucei Tb927.11.2300   eukaryotic peptide chain release factor subunit 1, putative
Trypanosoma congolense TcIL3000_0_54530   eukaryotic peptide chain release factor subunit 1, putative
Trypanosoma cruzi TcCLB.506127.110   eukaryotic peptide chain release factor subunit 1, putative
Toxoplasma gondii TGME49_304710   eukaryotic peptide chain release factor, putative
Theileria parva TP02_0750   eukaryotic peptide chain release factor, putative
Trichomonas vaginalis TVAG_020030   peptide chain release factor subunit, putative

Essentiality

PF3D7_0212300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.22.0012 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.22.0012 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.22.0012 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.22.0012 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T05H4.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_T05H4.6 Caenorhabditis elegans larval arrest wormbase
CELE_T05H4.6 Caenorhabditis elegans slow growth wormbase
CELE_T05H4.6 Caenorhabditis elegans sterile wormbase
YBR143C Saccharomyces cerevisiae inviable yeastgenome
TGME49_304710 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0212300 (Plasmodium falciparum), peptide chain release factor subunit 1, putative
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