Detailed view for PF3D7_0309000

Basic information

TDR Targets ID: 3590
Plasmodium falciparum, dual specificity protein phosphatase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.2954 | Length (AA): 575 | MW (Da): 67787 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00782   Dual specificity phosphatase, catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016791   phosphoric monoester hydrolase activity  
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0004725   protein tyrosine phosphatase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
289 433 1mkp () 207 347 23.00 0 0.97 0.59 -0.83
300 435 1zzw (A) 330 464 26.00 0.0000000000047 0.64 0.66 -1.26
285 435 2nt2 (A) 307 448 20.00 0.00011 0.51 0.473409 -0.75
287 431 2oud (A) 323 460 24.00 0.000067 1 0.512974 -0.26
315 430 2j16 (B) 85 191 29.00 0.00051 0.62 0.423939 0.25
342 431 3emu (A) 360 449 26.00 0.12 0.61 0.506722 -0.34
289 450 4ki9 (A) 27 186 23.00 0.0013 0.99 0.602939 -0.55
290 433 4jmk (A) 162 300 24.00 0.000069 0.68 0.600635 -0.85
290 441 2esb (A) 21 166 23.00 0.000058 0.98 0.608548 -0.69

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, Ring, Sporozoite, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 48 hs, Oocyst, Female Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127982)

Species Accession Gene Product
Arabidopsis thaliana AT4G18593   dual specificity protein phosphatase-like protein
Babesia bovis BBOV_III006280   dual specificity phosphatase, catalytic domain containing protein
Brugia malayi Bm1_24660   Dual specificity phosphatase, catalytic domain containing protein
Candida albicans CaO19.11879   potential dual specificity phosphatase similar to S. cerevisiae YVH1 (YIR026C) nitrogen starvation-induced tyrosine phosphatase
Candida albicans CaO19.4401   potential dual specificity phosphatase similar to S. cerevisiae YVH1 (YIR026C) nitrogen starvation-induced tyrosine phosphatase
Caenorhabditis elegans CELE_C24F3.2   Protein C24F3.2
Cryptosporidium hominis Chro.20172   dual-specificity protein phosphatase
Cryptosporidium parvum cgd2_1580   conserved hypothetical protein
Dictyostelium discoideum DDB_G0281963   hypothetical protein
Drosophila melanogaster Dmel_CG14211   MAPK Phosphatase 4
Echinococcus granulosus EgrG_000507100   dual specificity protein phosphatase 12
Entamoeba histolytica EHI_153220   dual specificity protein phosphatase, putative
Echinococcus multilocularis EmuJ_000507100   dual specificity protein phosphatase 12
Giardia lamblia GL50803_36315   Dual specificity protein phosphatase 12
Homo sapiens ENSG00000081721   dual specificity phosphatase 12
Leishmania braziliensis LbrM.09.1300   hypothetical protein, conserved
Leishmania donovani LdBPK_091310.1   hypothetical protein, conserved
Leishmania infantum LinJ.09.1310   hypothetical protein, conserved
Leishmania major LmjF.09.1250   hypothetical protein, conserved
Leishmania mexicana LmxM.09.1250   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_10521   dual specificity phosphatase
Mus musculus 102643029   dual specificity protein phosphatase 12-like
Mus musculus ENSMUSG00000026659   dual specificity phosphatase 12
Neospora caninum NCLIV_050170   hypothetical protein
Oryza sativa 4328898   Os02g0251700
Oryza sativa 4351431   Os12g0133700
Oryza sativa 4349715   Os11g0136800
Onchocerca volvulus OVOC2320  
Plasmodium berghei PBANKA_0407200   dual specificity protein phosphatase
Plasmodium falciparum PF3D7_0309000   dual specificity protein phosphatase
Plasmodium knowlesi PKNH_0833700   dual specificity protein phosphatase, putative
Plasmodium vivax PVX_119565   dual specificity protein phosphatase, putative
Plasmodium yoelii PY03455   putative dual-specificity protein phosphatase
Saccharomyces cerevisiae YIR026C   Yvh1p
Schistosoma japonicum Sjp_0059170   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma mansoni Smp_081510   hypothetical protein
Schmidtea mediterranea mk4.002546.00   LD31102p
Schmidtea mediterranea mk4.001002.16   LD31102p
Trypanosoma brucei gambiense Tbg972.11.14950   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.13390   hypothetical protein, conserved
Trypanosoma congolense TcIL3000.11.13760   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510359.140   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506755.130   hypothetical protein, conserved
Toxoplasma gondii TGME49_235890   dual-specificity protein phosphatase
Theileria parva TP02_0369   dual-specificity protein phosphatase, putative
Trichomonas vaginalis TVAG_306000   hyvh1 dual specificity phosphatase, putative
Trichomonas vaginalis TVAG_484590   hyvh1 dual specificity phosphatase, putative

Essentiality

PF3D7_0309000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4990 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.4990 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.4990 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.4990 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C24F3.2 Caenorhabditis elegans sterile wormbase
PBANKA_0407200 Plasmodium berghei Slow plasmo
TGME49_235890 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Pfal007889AAH;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pfal007889; Recombinant protein: truncated; Source: P falciparum; dual-specificity protein phosphatase, putative ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_0309000 (Plasmodium falciparum), dual specificity protein phosphatase
Title for this comment
Comment