Detailed view for TcCLB.511717.170

Basic information

TDR Targets ID: 37287
Trypanosoma cruzi, Metalloprotease M41 FtsH, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.4484 | Length (AA): 682 | MW (Da): 75710 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)
PF01434   Peptidase family M41

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0004222   metalloendopeptidase activity  
GO:0000166   nucleotide binding  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
168 429 1lv7 (A) 144 399 45.00 0 1 0.97 -0.91
172 679 2dhr (A) 149 596 42.00 0 1 1.12 -0.04
1 105 3pzd (A) 1557 1661 10.00 0 0.02 0.332659 -1.21
12 67 1b1u (A) 58 118 12.00 0 0.22 0.0518114 0.39
57 417 5c18 (A) 362 730 26.00 0.59 1 0.808425 0.11
138 360 5e7p (A) 443 662 37.00 0 1 0.80138 -0.41
171 679 2dhr (A) 148 596 41.00 0 1 1.01503 0.47
177 459 2qz4 (A) 307 530 52.00 0 1 0.607356 0.01
180 360 3d8b (A) 402 576 42.00 0 1 0.764796 -1.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_147363)

Species Accession Gene Product
Leishmania braziliensis LbrM.32.1670   ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania donovani LdBPK_321570.1   Metalloprotease M41 FtsH, putative
Leishmania infantum LinJ.32.1570   ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania major LmjF.32.1500   ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania mexicana LmxM.31.1500   ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Neospora caninum NCLIV_027270   cell division protein, putative
Trypanosoma brucei gambiense Tbg972.11.16520   metalloprotease, putative,cell division protein FtsH homologue, putative
Trypanosoma brucei Tb927.11.14730   Metalloprotease M41 FtsH, putative
Trypanosoma congolense TcIL3000.11.15020   Metalloprotease M41 FtsH, putative
Trypanosoma cruzi TcCLB.511717.170   Metalloprotease M41 FtsH, putative
Trypanosoma cruzi TcCLB.506223.80   Metalloprotease M41 FtsH, putative
Toxoplasma gondii TGME49_259260   membrane protein FtsH1

Essentiality

TcCLB.511717.170 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6360 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.6360 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_259260 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

54 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511717.170 (Trypanosoma cruzi), Metalloprotease M41 FtsH, putative
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