Detailed view for TcCLB.511813.20

Basic information

TDR Targets ID: 47812
Trypanosoma cruzi, ubiquitin-conjugating enzyme E2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.2506 | Length (AA): 223 | MW (Da): 25635 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00179   Ubiquitin-conjugating enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0019787   small conjugating protein ligase activity  
GO:0051246   regulation of protein metabolic process  
GO:0043687   post-translational protein modification  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
65 221 1y8x (A) 27 180 27.00 0 1 1.13 -1.33
68 212 2aak () 5 148 53.00 0 1 1.49 -2.25
20 210 3ceg (A) 4501 4708 26.00 0 1 1.1043 -0.34
65 213 2aak (A) 2 149 51.00 0 1 1.42416 -1.56
65 213 2yb6 (A) 2 149 51.00 0 1 1.43916 -1.71
66 209 4m1n (A) 2 152 35.00 0.00000039 1 1.20074 -1.75

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127153)

Species Accession Gene Product
Arabidopsis thaliana AT2G02760   ubiquitin-conjugating enzyme E2 A
Arabidopsis thaliana AT5G62540   ubiquitin-conjugating enzyme E2 3
Arabidopsis thaliana AT1G14400   ubiquitin-conjugating enzyme E2 1
Babesia bovis BBOV_II004660   ubiquitin-conjugating enzyme, putative
Babesia bovis BBOV_III003380   ubiquitin-conjugating enzyme family protein
Brugia malayi Bm1_31535   Ubiquitin-conjugating enzyme E2 1
Candida albicans CaO19_7195   hypothetical protein
Candida albicans CaO19.7195   3' exon of ubiquitin-conjugating enzyme gene
Caenorhabditis elegans CELE_C35B1.1   Protein UBC-1
Cryptosporidium hominis Chro.60479   ubiquitin-conjugating enzyme
Cryptosporidium parvum cgd6_4180   ubiquitin-conjugating enzyme
Cryptosporidium parvum cgd3_2670   protein with UBC domain, ubiquitin conjugating enzyme E2
Dictyostelium discoideum DDB_G0275787   ubiquitin-conjugating enzyme E2
Drosophila melanogaster Dmel_CG2013   Ubiquitin conjugating enzyme
Echinococcus granulosus EgrG_000834100   ubiquitin conjugating enzyme rad6
Echinococcus granulosus EgrG_000208000   ubiquitin conjugating enzyme E2 A
Entamoeba histolytica EHI_147700   ubiquitin-conjugating enzyme family protein
Echinococcus multilocularis EmuJ_000834100   ubiquitin conjugating enzyme rad6
Echinococcus multilocularis EmuJ_000208000   ubiquitin conjugating enzyme E2 A
Giardia lamblia GL50803_2876   UBC2, putative
Giardia lamblia GL50803_12950   Ubiquitin-conjugating enzyme E2-17 kDa
Giardia lamblia GL50803_15162   Ubiquitin-conjugating enzyme E2-17 kDa
Homo sapiens ENSG00000077721   ubiquitin-conjugating enzyme E2A
Homo sapiens ENSG00000119048   ubiquitin-conjugating enzyme E2B
Leishmania braziliensis LbrM.22.0550   ubiquitin-conjugating enzyme-like protein
Leishmania donovani LdBPK_220480.1   ubiquitin-conjugating enzyme-like protein
Leishmania infantum LinJ.22.0480   ubiquitin-conjugating enzyme-like protein
Leishmania major LmjF.22.0610   ubiquitin-conjugating enzyme-like protein
Leishmania mexicana LmxM.22.0610   ubiquitin-conjugating enzyme-like protein
Loa Loa (eye worm) LOAG_02518   ubiquitin-conjugating enzyme E2 1
Mus musculus ENSMUSG00000020390   ubiquitin-conjugating enzyme E2B
Mus musculus ENSMUSG00000016308   ubiquitin-conjugating enzyme E2A
Neospora caninum NCLIV_060020   Ubiquitin carrier protein (EC 6.3.2.-), related
Neospora caninum NCLIV_024450   ubiquitin-conjugating enzyme e2, putative
Oryza sativa 4334386   Os03g0791800
Oryza sativa 4342488   Os07g0166800
Oryza sativa 4337993   Os05g0182500
Plasmodium berghei PBANKA_0806000   ubiquitin-conjugating enzyme E2, putative
Plasmodium berghei PBANKA_1424900   ubiquitin-conjugating enzyme E2, putative
Plasmodium falciparum PF3D7_0319300   ubiquitin-conjugating enzyme E2, putative
Plasmodium falciparum PF3D7_0812600   ubiquitin-conjugating enzyme E2, putative
Plasmodium knowlesi PKNH_0821900   ubiquitin-conjugating enzyme E2, putative
Plasmodium knowlesi PKNH_1428000   ubiquitin-conjugating enzyme E2, putative
Plasmodium vivax PVX_123140   ubiquitin-conjugating enzyme E2, putative
Plasmodium vivax PVX_095270   ubiquitin-conjugating enzyme E2, putative
Plasmodium yoelii PY01792   ubiquitin-conjugating enzyme
Plasmodium yoelii PY00971   putative ubiquitin-conjugating enzyme
Saccharomyces cerevisiae YGL058W   E2 ubiquitin-conjugating protein RAD6
Schistosoma japonicum Sjp_0030560   ko:K10573 ubiquitin-conjugating enzyme E2 A, putative
Schistosoma japonicum Sjp_0057640   ko:K10573 ubiquitin-conjugating enzyme E2 A, putative
Schistosoma mansoni Smp_118220   ubiquitin-conjugating enzyme rad6
Schistosoma mansoni Smp_169440   ubiquitin-conjugating enzyme rad6
Schmidtea mediterranea mk4.000743.04   Ubiquitin-conjugating enzyme E2-17 kDa
Schmidtea mediterranea mk4.010792.00   Ubiquitin-conjugating enzyme E2 1
Trypanosoma brucei gambiense Tbg972.7.2830   ubiquitin-conjugating enzyme e2, putative,ubiquitin carrier protein, putative,ubiquitin-protein ligase, putative
Trypanosoma brucei Tb927.7.2540   ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_7_1880   ubiquitin-conjugating enzyme E2, putative
Trypanosoma congolense TcIL3000_0_55090   ubiquitin-conjugating enzyme E2, putative
Trypanosoma cruzi TcCLB.506859.10   ubiquitin-conjugating enzyme E2, putative
Trypanosoma cruzi TcCLB.511813.20   ubiquitin-conjugating enzyme E2, putative
Toxoplasma gondii TGME49_216130   ubiquitin conjugating enzyme E2, putative
Toxoplasma gondii TGME49_263490   ubiquitin conjugating enzyme E2, putative
Theileria parva TP03_0333   hypothetical protein, conserved
Theileria parva TP02_0288   ubiquitin-protein ligase, putative
Trichomonas vaginalis TVAG_085460   ubiquitin-conjugating enzyme E2r, putative

Essentiality

TcCLB.511813.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2540 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2540 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C35B1.1 Caenorhabditis elegans larval arrest wormbase
CELE_C35B1.1 Caenorhabditis elegans larval lethal wormbase
CELE_C35B1.1 Caenorhabditis elegans sterile wormbase
PBANKA_0806000 Plasmodium berghei Dispensable plasmo
TGME49_263490 Toxoplasma gondii Probably non-essential sidik
TGME49_216130 Toxoplasma gondii Probably non-essential sidik
TGME49_263490 Toxoplasma gondii Probably essential sidik
TGME49_216130 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511813.20 (Trypanosoma cruzi), ubiquitin-conjugating enzyme E2, putative
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