Detailed view for PF3D7_0916100

Basic information

TDR Targets ID: 4836
Plasmodium falciparum, thioredoxin 3
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5908 | Length (AA): 180 | MW (Da): 20941 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0015035   protein disulfide oxidoreductase activity  
GO:0045454   cell redox homeostasis  
GO:0006662   glycerol ether metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 132 1x5e (A) 5 114 16.00 0 1 1.07 -1.01
19 124 1xwa (A) 0 103 26.00 0 1 1.32 -2.28
56 178 3cxg (A) 9 131 92.00 0 1 1.85153 -2.21

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3CXG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_141346)

Species Accession Gene Product
Cryptosporidium hominis Chro.50325   thioredoxin
Cryptosporidium parvum cgd5_660   thioredoxin domain containing protein
Neospora caninum NCLIV_039420   thioredoxin, putative
Plasmodium berghei PBANKA_0817100   thioredoxin 3, putative
Plasmodium falciparum PF3D7_0916100   thioredoxin 3
Plasmodium knowlesi PKNH_0714100   thioredoxin 3, putative
Plasmodium vivax PVX_099235   thioredoxin 3, putative
Plasmodium yoelii PY00221   thioredoxin-related
Toxoplasma gondii TGME49_266620   thioredoxin domain-containing protein

Essentiality

PF3D7_0916100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_266620 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
response to oxidative stress (GO:0006979) increased sensitivity toward (PATO:0001549) bloodstream stage (PLO:0040) inferred from loss-of-function mutant phenotype (ECO:0000016) Plasmodium falciparum 118457   542659   542660   545056  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 12860402
growth (GO:0040007) normal (PATO:0000461) multi-cellular organism (CARO:0000012) bloodstream stage (PLO:0040) inferred from loss-of-function mutant phenotype (ECO:0000016) Plasmodium falciparum 118457   542659   542660   545056  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 12860402
Plasmodium falciparum 118457   542659   542660   545056  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 12860402 16307478

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0252 0.4853 1
0.0222 0.2796 1
0.0252 0.4637 1
0.0208 0.4577 1
0.0239 0.4487 1
0.0217 0.2783 0.766
0.0252 0.711 1
0.0252 0.711 1
0.0208 0.6515 1
0.0212 0.3881 1
0.0217 0.2786 1
0.0246 0.4594 1
0.0212 0.3751 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0916100 (Plasmodium falciparum), thioredoxin 3
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