Detailed view for TcCLB.511529.160

Basic information

TDR Targets ID: 49230
Trypanosoma cruzi, enoyl-CoA hydratase/isomerase family protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2706 | Length (AA): 362 | MW (Da): 40599 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF16113   Enoyl-CoA hydratase/isomerase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003860   3-hydroxyisobutyryl-CoA hydrolase activity  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 238 1sg4 (A) 2 235 15.00 0 0.78 0.92 -0.96
17 286 1wdk (A) 14 277 18.00 0 1 1.03 -0.78
6 356 4j2u (A) 0 338 29.00 0 1 1.37471 -0.87
9 351 3bpt (A) 35 381 38.00 0 1 1.48261 -0.97
12 346 3ju1 (A) 22 369 33.00 0 1 1.32691 -0.78
12 348 4hdt (A) 8 338 36.00 0 1 1.40944 -0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127836)

Species Accession Gene Product
Arabidopsis thaliana AT2G30660   probable 3-hydroxyisobutyryl-CoA hydrolase 3
Arabidopsis thaliana AT5G65940   beta-hydroxyisobutyryl-CoA hydrolase 1
Arabidopsis thaliana AT2G30650   probable 3-hydroxyisobutyryl-CoA hydrolase 2
Brugia malayi Bm1_41250   enoyl-CoA hydratase/isomerase family protein
Candida albicans CaO19.3029   Member of enoyl-coA hydratase/isomerase family
Candida albicans CaO19.10547   Member of enoyl-coA hydratase/isomerase family
Caenorhabditis elegans CELE_F09F7.4   Protein F09F7.4, isoform B
Dictyostelium discoideum DDB_G0267536   3-hydroxyisobutyryl-Coenzyme A hydrolase
Dictyostelium discoideum DDB_G0267600   enoyl-CoA hydratase/isomerase domain-containing protein
Dictyostelium discoideum DDB_G0287741   enoyl-CoA hydratase/isomerase domain-containing protein
Dictyostelium discoideum DDB_G0267598   enoyl-CoA hydratase/isomerase domain-containing protein
Drosophila melanogaster Dmel_CG5044   CG5044 gene product from transcript CG5044-RB
Homo sapiens ENSG00000198130   3-hydroxyisobutyryl-CoA hydrolase
Leishmania braziliensis LbrM.32.3920   enoyl-CoA hydratase/isomerase family protein, putative,3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Leishmania donovani LdBPK_323830.1   enoyl-CoA hydratase/isomerase family protein, putative
Leishmania infantum LinJ.32.3830   enoyl-CoA hydratase/isomerase family protein, putative,3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Leishmania major LmjF.32.3680   enoyl-CoA hydratase/isomerase family protein, putative,3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Leishmania mexicana LmxM.31.3680   enoyl-CoA hydratase/isomerase family protein, putative,3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Loa Loa (eye worm) LOAG_02686   enoyl-CoA hydratase/isomerase
Mycobacterium leprae ML2401   POSSIBLE ENOYL-CoA HYDRATASE ECHA9 (ENOYL HYDRASE) (UNSATURATED ACYL-CoA HYDRATASE) (CROTONASE)
Mus musculus ENSMUSG00000041426   3-hydroxyisobutyryl-Coenzyme A hydrolase
Mycobacterium tuberculosis Rv1071c   Possible enoyl-CoA hydratase EchA9 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
Mycobacterium ulcerans MUL_0209   3-hydroxyisobutyryl-CoA hydrolase
Neospora caninum NCLIV_048430   hypothetical protein
Oryza sativa 4351929   Os12g0264500
Plasmodium berghei PBANKA_1454400   3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Plasmodium falciparum PF3D7_1240000   3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Plasmodium knowlesi PKNH_1460100   3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Plasmodium vivax PVX_100950   3-hydroxyisobutyryl-coenzyme A hydrolase, putative
Plasmodium yoelii PY02220   similar to 3-hydroxyisobutyryl-Coenzyme A hydrolase, putative
Saccharomyces cerevisiae YDR036C   Ehd3p
Schmidtea mediterranea mk4.064011.03   3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Schmidtea mediterranea mk4.001430.00   3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Schmidtea mediterranea mk4.064011.02   3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Trypanosoma brucei gambiense Tbg972.11.18530   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma brucei Tb927.11.16480   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma congolense TcIL3000.11.16400   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma cruzi TcCLB.511529.170   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma cruzi TcCLB.511529.160   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma cruzi TcCLB.506727.90   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma cruzi TcCLB.506727.100   enoyl-CoA hydratase/isomerase family protein, putative
Toxoplasma gondii TGME49_224090   enoyl-CoA hydratase/isomerase family protein

Essentiality

TcCLB.511529.160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.8200 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8200 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.8200 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.8200 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F09F7.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F09F7.4 Caenorhabditis elegans slow growth wormbase
PBANKA_1454400 Plasmodium berghei Essential plasmo
TGME49_224090 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Crotonase (4.2.1.17 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Tcru023489AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru023489; Recombinant protein: full-length; Source: T cruzi; LD47223p ;

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511529.160 (Trypanosoma cruzi), enoyl-CoA hydratase/isomerase family protein, putative
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