Detailed view for PKNH_1338200

Basic information

TDR Targets ID: 527060
Plasmodium knowlesi, glutathione reductase, putative
Source Database / ID: 

pI: 7.769 | Length (AA): 561 | MW (Da): 64535 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02852   Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain
PF07992   Pyridine nucleotide-disulphide oxidoreductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016668   oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor  
GO:0016491   oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0050660   FAD binding  
GO:0055114   oxidation reduction  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

Glutathione metabolism (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
31 86 1d4d (A) 108 163 29.00 0 0.21 0.244522 1.36
48 556 1onf (A) 1 494 84.00 0 1 1.66911 0.09
238 374 2ywl (A) 2 144 15.00 0 0.93 0.376907 -0.6

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126785)

Species Accession Gene Product
Arabidopsis thaliana AT3G54660   glutathione reductase
Arabidopsis thaliana AT3G24170   glutathione-disulfide reductase
Babesia bovis BBOV_I002190   thiodoxin reductase, putative
Brugia malayi Bm1_04825   glutathione reductase
Brugia malayi Bm1_36410   Thioredoxin reductase
Candida albicans CaO19.11623   similar to S. cerevisiae GLR1 (YPL091W) glutathione oxidoreductase
Candida albicans CaO19.4147   similar to S. cerevisiae GLR1 (YPL091W) glutathione oxidoreductase
Caenorhabditis elegans CELE_C46F11.2   Protein GSR-1, isoform B
Caenorhabditis elegans CELE_ZK637.10   Protein TRXR-2
Cryptosporidium hominis Chro.20464   thioredoxin reductase
Cryptosporidium parvum cgd2_4320   thioredoxin reductase 1
Dictyostelium discoideum DDB_G0272754   glutathione reductase
Drosophila melanogaster Dmel_CG11401   thioredoxin reductase 2
Drosophila melanogaster Dmel_CG2151   Thioredoxin reductase-1
Escherichia coli b3500   glutathione oxidoreductase
Echinococcus granulosus EgrG_000222300   thioredoxin glutathione reductase
Echinococcus multilocularis EmuJ_000222300   thioredoxin glutathione reductase
Homo sapiens ENSG00000197763   thioredoxin reductase 3
Homo sapiens ENSG00000104687   glutathione reductase
Homo sapiens ENSG00000198431   thioredoxin reductase 1
Homo sapiens ENSG00000184470   thioredoxin reductase 2
Leishmania braziliensis LbrM.05.0350   trypanothione reductase
Leishmania donovani LdBPK_050350.1   trypanothione reductase
Leishmania infantum LinJ.05.0350   trypanothione reductase
Leishmania major LmjF.05.0350   trypanothione reductase
Leishmania mexicana LmxM.05.0350   trypanothione reductase
Loa Loa (eye worm) LOAG_01116   glutathione reductase
Loa Loa (eye worm) LOAG_02317   thioredoxin reductase
Mus musculus ENSMUSG00000031584   glutathione reductase
Mus musculus ENSMUSG00000075704   thioredoxin reductase 2
Mus musculus ENSMUSG00000020250   thioredoxin reductase 1
Mus musculus ENSMUSG00000000811   thioredoxin reductase 3
Mycobacterium tuberculosis Rv2855   NADPH-dependent mycothiol reductase Mtr
Neospora caninum NCLIV_053860   MGC84926 protein, related
Neospora caninum NCLIV_063590   Glutathione reductase, related
Oryza sativa 9270365   Os10g0415112
Oryza sativa 4331112   Os02g0813500
Oryza sativa 4348623   Os10g0415300
Oryza sativa 4331719   Os03g0163300
Plasmodium berghei PBANKA_0824700   thioredoxin reductase, putative
Plasmodium berghei PBANKA_1023400   glutathione reductase, putative
Plasmodium falciparum PF3D7_0923800   thioredoxin reductase
Plasmodium falciparum PF3D7_1419800   glutathione reductase
Plasmodium knowlesi PKNH_0721800   thioredoxin reductase, putative
Plasmodium knowlesi PKNH_1338200   glutathione reductase, putative
Plasmodium vivax PVX_099600   thioredoxin reductase, putative
Plasmodium vivax PVX_085490   glutathione reductase, putative
Plasmodium yoelii PY01204   glutathione reductase
Plasmodium yoelii PY02397   thioredoxin reductase
Saccharomyces cerevisiae YPL091W   glutathione-disulfide reductase GLR1
Schistosoma japonicum Sjp_0021180   ko:K00384 thioredoxin reductase (NADPH) [EC1.8.1.9], putative
Schmidtea mediterranea mk4.000203.07   Probable glutathione reductase 2
Schmidtea mediterranea mk4.000203.06  
Schmidtea mediterranea mk4.001663.03  
Trypanosoma brucei gambiense Tbg972.10.12680   trypanothione reductase, putative
Trypanosoma brucei Tb927.10.10390   trypanothione reductase
Trypanosoma congolense TcIL3000_10_8870   trypanothione reductase, putative
Trypanosoma cruzi TcCLB.503555.30   trypanothione reductase, putative
Toxoplasma gondii TGME49_309730   thioredoxin reductase
Theileria parva TP03_0110   thioredoxin reductase, putative

Essentiality

PKNH_1338200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu2902 Mycobacterium tuberculosis essential nmpdr
Tb927.10.10390 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.10390 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.10390 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.10390 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3500 Escherichia coli non-essential goodall
PBANKA_1023400 Plasmodium berghei Dispensable plasmo
TGME49_309730 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum glutathione reductase Compounds References
Schistosoma mansoni Thioredoxin glutathione reductase Compounds References
Leishmania infantum trypanothione reductase Compounds References
Homo sapiens thioredoxin reductase 3 Compounds References
Crithidia fasciculata Trypanothione reductase Compounds References
Rattus norvegicus Thioredoxin reductase 1, cytoplasmic Compounds References
Leishmania donovani trypanothione reductase Compounds References
Homo sapiens thioredoxin reductase 2 Compounds References
Plasmodium falciparum thioredoxin reductase Compounds References
Rattus norvegicus Thioredoxin reductase 2, mitochondrial Compounds References
Trypanosoma cruzi trypanothione reductase, putative Compounds References
Saccharomyces cerevisiae glutathione-disulfide reductase GLR1 Compounds References
Trypanosoma brucei gambiense trypanothione reductase, putative Compounds References
Leishmania major trypanothione reductase Compounds References
Homo sapiens thioredoxin reductase 1 Compounds References
Rattus norvegicus Glutathione reductase Compounds References
Trypanosoma brucei trypanothione reductase Compounds References
Homo sapiens glutathione reductase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 510 aa 20.5% 521 aa Compounds References
Trypanosoma cruzi trypanothione reductase, putative 189 aa 35.3% 201 aa Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 550 aa 19.3% 538 aa Compounds References
Mycobacterium tuberculosis Probable thioredoxin reductase TrxB2 (TRXR) (TR) 335 aa 19.7% 366 aa Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 510 aa 20.5% 521 aa Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 550 aa 19.1% 551 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PKNH_1338200 (Plasmodium knowlesi), glutathione reductase, putative
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