Detailed view for Tb927.5.580

Basic information

TDR Targets ID: 20055
Trypanosoma brucei, prefoldin subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.8119 | Length (AA): 135 | MW (Da): 15578 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01920   Prefoldin subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0016272   prefoldin complex  
GO:0051082   unfolded protein binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 134 2zqm (A) 1 113 28.00 0 0.5 1.07399 0.06
1 135 2yfa (A) 138 265 10.00 0 0.06 1.1494 -1.51
2 134 4tql (A) 10 142 20.00 0.25 0.09 1.24599 -0.88
2 134 1z2c (D) 237 366 14.00 0 0.42 1.14759 -0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127549)

Species Accession Gene Product
Arabidopsis thaliana AT1G29990   prefoldin 6
Babesia bovis BBOV_IV004800   prefoldin subunit 6, putative
Brugia malayi Bm1_30720   KE2 family protein
Caenorhabditis elegans CELE_F21C3.5   Protein PFD-6
Cryptosporidium hominis Chro.30152   hypothetical protein
Cryptosporidium parvum cgd3_1200   possible prefoldin-related protein
Dictyostelium discoideum DDB_G0286147   prefoldin beta-like domain containing protein
Drosophila melanogaster Dmel_CG7770   CG7770 gene product from transcript CG7770-RB
Echinococcus granulosus EgrG_000132500   prefoldin subunit 6
Entamoeba histolytica EHI_155420   prefoldin subunit 6, putative
Echinococcus multilocularis EmuJ_000132500   prefoldin subunit 6
Giardia lamblia GL50803_112870   Hypothetical protein
Homo sapiens ENSG00000204220   prefoldin subunit 6
Leishmania braziliensis LbrM.05.1190   prefoldin subunit, putative
Leishmania donovani LdBPK_051190.1   prefoldin subunit, putative
Leishmania infantum LinJ.05.1190   prefoldin subunit, putative
Leishmania major LmjF.05.1200   prefoldin subunit, putative
Leishmania mexicana LmxM.05.1200   prefoldin subunit, putative
Loa Loa (eye worm) LOAG_03329   hypothetical protein
Mus musculus ENSMUSG00000024309   prefoldin subunit 6
Neospora caninum NCLIV_064340   prefoldin subunit, putative
Oryza sativa 4328867   Os02g0246300
Plasmodium berghei PBANKA_1111600   prefoldin subunit 6, putative
Plasmodium falciparum PF3D7_0512000   prefoldin subunit 6, putative
Plasmodium knowlesi PKNH_1021600   prefoldin subunit 6, putative
Plasmodium vivax PVX_080640   prefoldin subunit 6, putative
Saccharomyces cerevisiae YLR200W   Yke2p
Schistosoma japonicum Sjp_0002020   ko:K04798 prefoldin beta subunit, putative
Schistosoma mansoni Smp_027880   hypothetical protein
Schmidtea mediterranea mk4.000797.00   Prefoldin subunit 6
Trypanosoma brucei gambiense Tbg972.5.610   prefoldin subunit, putative
Trypanosoma brucei Tb927.5.580   prefoldin subunit, putative
Trypanosoma congolense TcIL3000_5_280   prefoldin subunit, putative
Trypanosoma cruzi TcCLB.507867.70   prefoldin subunit, putative
Trypanosoma cruzi TcCLB.510629.430   prefoldin subunit, putative
Toxoplasma gondii TGME49_248370   prefoldin subunit 6, putative
Theileria parva TP01_0267   hypothetical protein, conserved
Trichomonas vaginalis TVAG_277370   conserved hypothetical protein

Essentiality

Tb927.5.580 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.580 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.580 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.580 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.580 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F21C3.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F21C3.5 Caenorhabditis elegans larval arrest wormbase
CELE_F21C3.5 Caenorhabditis elegans slow growth wormbase
TGME49_248370 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus MHC class II Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0107 0.3271 1
0.0062 0.352 0.7659
0.0038 0.5 0.5
0.0038 0.5 0.5
0.0037 0.5 0.5
0.0062 1 0.5
0.0176 1 0.5
0.0062 1 0.5
0.0037 0.5 0.5
0.0062 1 0.5
0.0102 0.3181 1
0.0108 0.3248 1
0.0061 1 1
0.0108 1 1
0.0061 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru015727AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru015727; Recombinant protein: full-length; Source: T brucei; prefoldin subunit, putative ;

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.5.580 (Trypanosoma brucei), prefoldin subunit, putative
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