Detailed view for TcCLB.503855.40

Basic information

TDR Targets ID: 38687
Trypanosoma cruzi, calpain-like cysteine peptidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7281 | Length (AA): 850 | MW (Da): 95218 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF09149   Domain of unknown function (DUF1935)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
140 251 1r75 (A) 13 132 39.00 0 0.98 0.52 -0.56
141 234 1r75 (A) 14 115 45.00 0 0.97 0.68 -0.85
265 614 2g8j (A) 33 351 29.00 0 1 0.59 -0.37
268 843 1df0 (A) 23 596 20.00 0 1 0.64 0.88
133 251 2fe0 (A) 6 126 47.00 0 1 0.7187 -0.31
140 251 1r75 (A) 13 132 38.00 0 0.98 0.605165 -0.49
278 619 3bow (A) 33 346 28.00 0 1 0.653753 -0.17
278 775 1qxp (B) 33 539 24.00 0 1 0.601282 1.09
278 608 2p0r (A) 30 328 35.00 0 1 0.611712 0.5
794 849 1dtl (A) 29 82 9.00 0.94 0 0.0765823 0.37

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_135138)

Species Accession Gene Product
Leishmania braziliensis LbrM.20.5380   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.04.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_040430.1   calpain-like cysteine peptidase, putative
Leishmania infantum LinJ.20.1220   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.04.0430   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.20.1210   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.20.1185   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.20.1180   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.04.0450   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.20.1185   calpain-like cysteine peptidase, putative
Leishmania mexicana LmxM.20.1180   calpain-like cysteine peptidase
Leishmania mexicana LmxM.04.0450   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.9.4110   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.1.1230   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.9.7540   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.1.2100   Calpain-like protein 1.1
Trypanosoma congolense TcIL3000_0_54930   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma cruzi TcCLB.503855.40   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.508999.190   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma cruzi TcCLB.510957.9   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.510337.20   cysteine peptidase, Clan CA, family C2, putative

Essentiality

TcCLB.503855.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.2100 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.2100 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.2100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.2100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.5550 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 414 aa 29.8% 413 aa Compounds References
Leishmania major calpain family cysteine protease-like protein 705 aa 30.2% 726 aa Compounds References
Leishmania major cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative 723 aa 30.8% 704 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 732 aa 33.2% 614 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 743 aa 31.7% 775 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 687 aa 36.0% 737 aa Compounds References
Leishmania major small myristoylated protein-1 131 aa 52.3% 109 aa Compounds References
Leishmania major small myristoylated protein-3, putative 115 aa 41.3% 109 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 754 aa 24.8% 738 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0807 1 1
0.0772 0.7603 0.8337
0.0884 1 1
0.1905 1 1
0.0811 0.8272 1
0.0799 1 1
0.0815 1 1
0.1045 0.2596 0.2587
0.0907 0.3426 0.7416
0.0792 0.8844 1
0.0848 0.4027 1
0.0826 1 1
0.0931 0.6669 0.8653
0.0842 0.91 1
0.1707 1 1
0.0443 1 1
0.0927 0.2694 1
0.0497 0.4878 0.4878
0.0907 0.2907 0.2907
0.1439 1 1
0.0851 1 1
0.0974 1 1
0.0889 1 1
0.1045 0.2596 0.2587
0.0772 0.6292 0.6882
0.0883 0.9132 1
0.0497 0.4878 0.4878
0.0829 0.5956 0.769
0.0803 0.2876 1
0.0912 0.3593 1
0.1891 0.7283 1
0.0929 1 1
0.0804 0.323 0.6432
0.093 0.4006 0.4687
0.1891 0.7276 1
0.0912 0.3254 1
0.0734 0.2647 1
0.1806 1 1
0.1806 1 1
0.0995 1 1
0.1792 1 1
0.0868 0.6975 1
0.0871 1 1
0.2003 1 1
0.0847 1 1
0.1044 0.3277 0.3277
0.0961991 0.711247 1
0.0846 0.3022 0.3017
0.0902 1 1
0.0803 0.575 1
0.0884 0.3557 0.5378
0.0934 0.9636 1
0.0862 0.3249 1
0.0803 0.9137 1
0.0848 0.8957 1
0.0804 0.323 0.6432
0.0815 1 1
0.0873 1 1
0.0927 0.2694 1
0.039 0.2582 1
0.0789 0.7423 1
0.0537 0.623 1
0.0903 0.921 1
0.0497 0.4878 0.4878
0.0977 1 1
0.0934 0.9798 1
0.0902 1 1
0.0968 1 1
0.0884 1 1
0.1086 0.828 1
0.039 0.3921 1
0.0811 0.2665 1
0.0884 0.5478 1
0.0995 0.2618 0.2919
0.0867 1 1
0.0935 0.9848 1
0.0806 1 1
0.1891 0.7276 1
0.0858 1 1
0.0974 0.2547 0.5053
0.0584 0.3017 0.547
0.0826 1 1
0.0995 0.2618 0.2919
0.0855 1 1
0.0964 1 1
0.0886 1 1
0.0811 0.2665 1
0.0842 0.91 1
0.1905 1 1
0.0876 0.6852 1
0.0977 1 1
0.091 0.4857 1
0.0934 0.9798 1
0.0929 1 1
0.0761 0.6275 1
0.0884 1 1
0.0848 0.887 1
0.0821 1 1
0.1792 0.7283 1
0.0774 0.2962 0.2952
0.1496 0.3111 1
0.0995 1 1
0.093 0.3138 0.3132
0.0912 0.3281 1
0.0878 0.2535 1
0.0848 0.6006 1
0.0776 0.8501 1
0.1989 1 1
0.1608 0.7283 1
0.0765 0.7745 0.7738
0.1439 0.7286 1
0.0982 0.2596 0.2585
0.0901 0.4183 0.7533
0.0912 0.3281 1
0.0835 0.2598 1
0.0922 1 1
0.0862 0.3187 1
0.0819 0.3347 0.7473
0.0803 0.4345 1
0.089 0.3254 0.3925
0.0806 1 1
0.0875 0.4445 0.8188
0.0497 0.4878 0.4878
0.1891 0.7276 1
0.0772 0.6292 0.6882
0.0847 0.4873 1
0.071 0.5552 1
0.0799 0.2919 1
0.189 1 1
0.0721 1 1
0.0887 0.3574 0.3567
0.0839 0.4446 0.4441
0.0862 0.3249 1
0.0974 0.2547 0.5053
0.0848 0.8957 1
0.0833 1 1
0.0878 0.2535 1
0.0812 0.6045 0.6747
0.0884 0.4473 0.446
0.0929 1 1
0.0916 0.3025 0.3504
0.0803 0.575 1
0.0942 0.5998 1
0.1538 0.7298 1
0.0845 0.6701 1
0.099 0.2885 0.2885
0.0828 1 1
0.0785 0.8806 1
0.2003 1 1
0.0752 0.8206 0.8206
0.0837 0.7377 1
0.089 0.2838 0.2834
0.0827 1 1
0.0844 1 1
0.0776 0.8501 1
0.0848 0.6006 1
0.0497 0.4878 0.4878
0.1008 1 1
0.0912 0.376 0.3759
0.092 1 1
0.193072 1 1
0.189 1 1
0.082 0.2815 0.2814
0.0779 0.9333 1
0.0855 1 1
0.0844 0.3408 0.3408
0.0957 1 1
0.0797 0.9228 1
0.045 1 1
0.0917 0.2984 0.2975
0.201 1 1
0.0912 1 1
0.0772 0.7613 1
0.081 0.592 0.939
0.189 1 1
0.0811 0.8272 1
0.0884 0.5444 1
0.0857 0.2889 0.2886
0.1792 0.7283 1
0.1008 1 1
0.08 0.3162 0.4064
0.0865 0.3964 0.3954
0.0912 0.3281 1
0.0854 1 1
0.0833 1 1
0.0938 1 1
0.0982 0.2596 0.2585
0.0868 0.6975 1
0.0903 1 1
0.1042 0.3194 0.3193
0.0819 0.8779 1
0.0902 0.3687 0.3676
0.1008 1 1
0.0855 0.4951 1
0.085 0.4543 0.4537
0.093 0.4006 0.4687
0.1989 1 1
0.0803 0.4345 1
0.0977 1 1
0.0995 0.4621 1
0.0864 0.9524 1
0.0497 0.4878 0.4878
0.0969 0.5878 1
0.0815 0.4807 1
0.0645 1 1
0.0985 0.6125 1
0.0849 0.4343 0.4333
0.164447 1 1
0.1989 1 1
0.0858 1 1
0.0497 0.4878 0.4878
0.084 1 1
0.1792 0.7283 1
0.0945 0.4992 1
0.093 0.4006 0.4687
0.0958 0.9998 1
0.0897 0.9941 1
0.0924 0.5378 0.5411
0.0883 0.9132 1
0.0852 0.5692 1
0.0772 0.7608 0.9735
0.0638 1 1
0.0803 0.9137 1
0.0886 1 1
0.1045 0.2596 0.2587
0.0995 0.2618 0.2919
0.0349 0.4233 1
0.0638 1 1
0.0925 1 1
0.0975 1 1
0.0855 1 1
0.1109 0.2882 0.3005
0.087 0.2905 0.29
0.0537 0.6482 1
0.0975 1 1
0.1891 0.7276 1
0.0785 0.4911 0.7653
0.0584 0.3017 0.547
0.0829 0.328 0.4919
0.0928 0.3007 0.3083
0.0806 1 1
0.0764 0.8752 1
0.0884 0.3557 0.5378
0.0835 0.7054 1
0.0846 1 1
0.1059 0.2888 0.3334
0.0824 1 1
0.0878 0.8921 1
0.0957 1 1
0.0809 1 1
0.0919 0.3462 1
0.0871 1 1
0.0822 0.5335 0.9008
0.1059 0.2888 0.3334
0.0982 0.2596 0.2585
0.0826 0.8051 1
0.09 0.4109 0.4109
0.0984 0.3305 0.3305
0.1013 0.3183 0.3179
0.0884 1 1
0.0812 1 1
0.0765 0.7745 0.7738
0.0901 0.4183 0.7533
0.078 0.7592 1
0.0995 0.4621 1
0.0811 0.2665 1
0.164447 1 1
0.0772 0.6292 0.6882
0.0797 1 1
0.0874 0.4365 0.4361
0.0811 0.2665 1
0.0942 0.7508 1
0.0846 1 1
0.0967 1 1
0.0897 1 1
0.031 0.3303 0.6313
0.1905 1 1
0.0763 0.2733 0.2727
0.0809 1 1
0.0848 0.3166 0.892
0.0848 0.8957 1
0.0848 0.6006 1
0.0818 0.3004 0.8989
0.0887 0.9024 1
0.0792 0.8844 1
0.0825 0.5382 0.9005
0.0955 1 1
0.0985 1 1
0.0835 1 1
0.0679 0.4738 1
0.0876 0.9978 1
0.0797 0.5209 0.6513
0.0825 0.3547 1
0.0995 0.2618 0.2919
0.0896 0.3841 0.383
0.092 0.2968 0.2962
0.189 1 1
0.0911 0.4582 0.4572
0.0995 1 1
0.087 1 1
0.0699 0.3804 1
0.0638 1 1
0.0958 0.7454 1
0.0926 0.2807 0.2795
0.0809 1 1
0.1792 1 1
0.0974 0.2547 0.5053
0.0844 1 1
0.0848 0.4027 1
0.0857 0.2889 0.2886
0.0848 1 1
0.0884 1 1
0.0772 0.6292 0.6882
0.1891 0.7283 1
0.0949 0.3805 0.4006
0.0866 0.8734 1
0.0789 0.7423 1
0.1989 1 1
0.0766 0.7977 1
0.0819 0.3347 0.7473
0.0985 1 1
0.0982 0.2596 0.2585
0.092 1 1
0.0863 0.2735 1
0.0803 0.9137 1
0.0803 0.2876 1
0.0972 1 1
0.1045 0.2596 0.2587
0.0931 0.6669 0.8653
0.0826 1 1
0.0802 0.7948 0.8805
0.093 0.4006 0.4687
0.0789 0.7423 1
0.0788 0.5961 1
0.0837 0.7377 1
0.0805 1 1
0.0852 0.5692 1
0.0818 0.3004 0.8989
0.0632 1 1
0.0435 0.4305 1
0.0874 0.975 1
0.071 0.5552 1
0.1045 0.2596 0.2587
0.075 0.6083 1
0.0868 0.6975 1
0.0912 0.3593 1
0.0772 0.7608 0.9735
0.0972 0.4318 0.431
0.0927 0.3963 1
0.0497 0.4878 0.4878
0.0803 0.5392 1
0.0924 0.5378 0.5411
0.0884 0.3557 0.5378
0.1203 0.2771 0.2778
0.0942 0.7508 1
0.0977 1 1
0.0803 0.751 1
0.0959 1 1
0.0892 0.6143 1
0.0907 0.3426 0.7416
0.1989 1 1
0.0903 0.9937 1
0.0884 0.3526 1
0.0603 0.4489 1
0.0995 0.2618 0.2919
0.0902 1 1
0.0763 0.2733 0.2727
0.0443 1 1
0.1439 0.7286 1
0.0975 1 1
0.0904 0.307 0.3062
0.0848 0.6006 1
0.0868 0.6975 1
0.2003 1 1
0.0826 1 1
0.1707 1 1
0.0772 0.6292 0.6882
0.0974 0.2547 0.5053
0.0763 0.2733 0.2727
0.0863 0.6087 0.6219
0.0902 1 1
0.1008 1 1
0.0974 0.2547 0.5053
0.0937 1 1
0.0833 1 1
0.084 1 1
0.0799 1 1
0.091 0.4857 1
0.0912 0.3281 1
0.0732 0.8122 0.8114
0.0871 0.3076 0.3075
0.0843 0.5787 1
0.0991 0.6169 1
0.0894 1 1
0.0836 0.4265 0.4249
0.1059 0.2888 0.3334
0.1439 0.7251 1
0.0912 0.2962 0.295
0.0884 0.5444 1
0.1806 1 1
0.0808 0.4458 0.4451
0.0995 0.2618 0.2919
0.0831 0.5389 0.902
0.091 0.4857 1
0.0766 0.7977 1
0.0408 0.2712 1
0.1538 0.7298 1
0.092 0.2968 0.2962
0.0942 0.3697 1
0.100192 1 1
0.0815 0.4807 1
0.0862 0.4806 0.523
0.0821 1 1
0.0967 1 1
0.0491 0.2608 0.7464
0.0803 0.5392 1

Assayability

Assay information

  • Assay for Cathepsin B (3.4.22.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

20 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503855.40 (Trypanosoma cruzi), calpain-like cysteine peptidase, putative
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