Detailed view for Tb927.7.7480

Basic information

TDR Targets ID: 19117
Trypanosoma brucei, trans-sialidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9631 | Length (AA): 816 | MW (Da): 90234 | Paralog Number: 5

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13859   BNR repeat-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004308   exo-alpha-sialidase activity  
GO:0009405   pathogenesis  

Metabolic Pathways

Other glycan degradation (KEGG)
Sphingolipid metabolism (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
65 467 4bbw (A) 143 526 21.00 0 0.81 0.549273 1.02
112 734 1ms9 (A) 8 627 41.00 0 1 1.29358 -0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130137)

Species Accession Gene Product
Schmidtea mediterranea mk4.000299.20  
Trypanosoma brucei gambiense Tbg972.7.8790   trans-sialidase, putative
Trypanosoma brucei gambiense Tbg972.7.7980   trans-sialidase, putative
Trypanosoma brucei gambiense Tbg972.8.7610   trans-sialidase, putative,neuraminidase, putative
Trypanosoma brucei gambiense Tbg972.5.850   trans-sialidase, putative,neuraminidase, putative
Trypanosoma brucei gambiense Tbg972.11.9090   trans-sialidase, putative
Trypanosoma brucei Tb927.7.6850   trans-sialidase
Trypanosoma brucei Tb927.7.7480   trans-sialidase, putative
Trypanosoma brucei Tb927.7.6830   trans-sialidase, putative
Trypanosoma brucei Tb927.8.7350   trans-sialidase, putative
Trypanosoma brucei Tb927.5.640   trans-sialidase, putative
Trypanosoma brucei Tb927.8.7340   trans-sialidase, putative
Trypanosoma congolense TcIL3000_0_42170   trans-sialidase
Trypanosoma congolense TcIL3000_0_01140   trans-sialidase
Trypanosoma congolense TcIL3000_0_35120   trans-sialidase
Trypanosoma congolense TcIL3000_7_5530   trans-sialidase
Trypanosoma congolense TcIL3000_0_09500   trans-sialidase
Trypanosoma congolense TcIL3000_0_17070   trans-sialidase
Trypanosoma congolense TcIL3000_0_12970   trans-sialidase
Trypanosoma congolense TcIL3000_0_33860   trans-sialidase
Trypanosoma congolense TcIL3000_0_07070   trans-sialidase
Trypanosoma cruzi TcCLB.507085.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.510055.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.508343.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.505931.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.509495.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508089.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.509817.50   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.503993.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508501.320   trans-sialidase, putative
Trypanosoma cruzi TcCLB.510787.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.506975.80   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.507979.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508859.118   trans-sialidase, putative
Trypanosoma cruzi TcCLB.401569.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.511323.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508857.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.506895.80   trans-sialidase, putative
Trypanosoma cruzi TcCLB.509481.10   trans-sialidase, putative

Essentiality

Tb927.7.7480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.7480 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.7480 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.7480 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.7480 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.6850 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6850 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.6850 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6850 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Clostridium perfringens Sialidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0031 0.8786 0.8786
0.0035 1 0.5
0.035 0.2815 1
0.0031 0.6594 0.4741
0.0029 0.6234 0.6159
0.0085 0.2595 1
0.003 0.6333 0.6265
0.003 0.4547 0.574
0.0324 0.2704 0.6454
0.0031 0.2776 0.4998
0.003 0.6974 0.5515
0.003 0.6594 0.4742
0.0034 1 1
0.0355 0.2766 0.5
0.0296 0.2628 1
0.0123 0.6504 1
0.0033 0.7063 0.5942
0.003 0.4431 0.5702
0.0031 0.4669 0.5777
0.003 0.6579 0.4651
0.0031 0.2776 0.4998
0.0307 0.2501 1
0.0324 0.2704 0.6454
0.0144 0.3728 0.7803
0.003 0.268 0.4938
0.0034 0.3485 0.5938
0.003 0.4287 0.5659
0.0031 0.6594 0.4741
0.003 0.6974 0.5515
0.0031 0.6593 0.474
0.0031 0.281 0.5018
0.003 0.4432 0.5702
0.0306 0.2516 1
0.003 0.4287 0.5658
0.003 0.4408 0.5695
0.0324 0.2547 1
0.0031 0.2812 0.5019
0.0144 0.3728 0.7803
0.0028 0.3979 0.5539
0.0031 0.2619 0.4898
0.0028 0.669 0.441
0.0031 0.2622 0.489
0.0029 0.6512 0.4356
0.003 0.7074 0.7074
0.0029 0.6512 0.4357
0.0033 0.7063 0.5942
0.0029 0.6511 0.4352

Assayability

Assay information

  • Assay for Neuraminidase Stopped Spectrophotometric Rate Determination (3.2.1.18 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.7.7480 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

261 literature references were collected for this gene.

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Gene identifier Tb927.7.7480 (Trypanosoma brucei), trans-sialidase, putative
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